rna-isolation-purification-cells-primary-rat-cardiac-fibroblasts

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human umbilical artery endothelial cells

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human pulmonary artery endothelial cells

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human lung microvascular endothelial cells

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human dermal microvascular endothelial cells

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human coronary artery endothelial cells

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human aortic smooth muscle cells

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary bovine pulmonary artery endothelial cells

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary bovine coronary artery endothelial cells

Isolating RNA from tissues and paraffin-embedded tissue samples can be challenging due to cross-linking of biomolecules and fragmented nucleic acids. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in presence of RNAse inhibitors. The homogenization process should be carried out on dry ice to maintain the integrity of RNA.