Get tips on using CpGrich to perform Protein Expression Eukaryotic cells - CHO EGFP
Get tips on using ISOGEN to perform RNA isolation / purification Cells - immortalized CHO
Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.
Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.
Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.
Get tips on using pDDGFP2_A2AR to perform Protein Expression Eukaryotic cells - S. cerevisiae A2AR
Get tips on using pSynInsCPOT to perform Protein Expression Eukaryotic cells - S. cerevisiae Insulin
Get tips on using pSynInsPOT to perform Protein Expression Eukaryotic cells - S. cerevisiae Insulin
Get tips on using pSynAmyCPOT to perform Protein Expression Eukaryotic cells - S. cerevisiae Amylase
Get tips on using pSynAmyPOT to perform Protein Expression Eukaryotic cells - S. cerevisiae Amylase
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