rna-isolation-purification-cells-primary-rat-cardiac-fibroblasts

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RNA-Seq is a method to sequence RNA by applying Next Generation Sequencing (NGS). The quality of RNA is critical for the success of RNA-Seq. The integrity of RNA is measured by the RNA integrity number (RIN). RIN is computed from RNA electrophoresis and electropherogram profiles (the peak area of the 28S rRNA should be approximately twice the peak area of the 18S rRNA). If you get the RIN value lower than 7, the possibility of getting the low quality of RNA-seq data is high. To get a high quality RNA, it is better to work with fresh samples or snap-freeze the tissues in liquid nitrogen as quickly as possible and store them at -80°C until further use. Make sure designated areas and all your filter tips, microfuge tubes, plastic, and glassware are RNase-free.

RNA RNA sequencing Rat A7R5

RNA-Seq is a method to sequence RNA by applying Next Generation Sequencing (NGS). The quality of RNA is critical for the success of RNA-Seq. The integrity of RNA is measured by the RNA integrity number (RIN). RIN is computed from RNA electrophoresis and electropherogram profiles (the peak area of the 28S rRNA should be approximately twice the peak area of the 18S rRNA). If you get the RIN value lower than 7, the possibility of getting the low quality of RNA-seq data is high. To get a high quality RNA, it is better to work with fresh samples or snap-freeze the tissues in liquid nitrogen as quickly as possible and store them at -80°C until further use. Make sure designated areas and all your filter tips, microfuge tubes, plastic, and glassware are RNase-free.

RNA RNA sequencing Rat PC12

Get tips on using NucleoSpin® RNA to perform RNA isolation / purification Tissue - Rat Lymph node

Products Macherey Nagel NucleoSpin® RNA

Get tips on using NucleoSpin® RNA to perform RNA isolation / purification Tissue - Rat Bone marrow

Products Macherey Nagel NucleoSpin® RNA

Get tips on using Gentra Puregene Cell Kit to perform DNA isolation / purification Cells - Primary cells Human primary keratinocytes

Products Qiagen Gentra Puregene Cell Kit

Get tips on using ARCTURUS® PicoPure® DNA Extraction Kit to perform RNA isolation / purification Cells - primary human melanocytes

Products Thermo Fisher Scientific ARCTURUS® PicoPure® DNA Extraction Kit

Get tips on using Absolutely RNA FFPE Kit to perform RNA isolation / purification Tissue - Rat Kidney

Products Agilent Technologies Absolutely RNA FFPE Kit

Get tips on using NucleoSpin® RNA/Protein to perform RNA isolation / purification Tissue - Rat Hippocampus

Products Macherey Nagel NucleoSpin® RNA/Protein

Transfection is a powerful technique that enables the study of the function of genes and gene products in cells. Based on the nature of experiments, we may need a stable DNA transfection in cells for persistent gain-of-function or loss-of-function of the target gene. For stable transfection, integration of a DNA vector into the chromosome is crucial which requires selective screening and clonal isolation. By carefully selecting a viral delivery system and related reagents we can ensure safe and highly-efficient delivery of expression constructs for high-level constitutive or inducible expression in any mammalian cell type.

DNA DNA transfection Mammalian cells Primary cells Human pulmonary artery smooth muscle cells (HPASMC)

Get tips on using miRCURY RNA Isolation Kit to perform RNA isolation / purification Cells - immortalized A549

Products Exiqon miRCURY RNA Isolation Kit

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