rna-isolation-purification-cells-primary-rat-cardiac-fibroblasts

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miRCURY RNA Isolation Kit Product

Get tips on using miRCURY RNA Isolation Kit to perform RNA isolation / purification Cells - immortalized A549

Products Exiqon miRCURY RNA Isolation Kit

miRCURY RNA Isolation Kit Product

Get tips on using miRCURY RNA Isolation Kit to perform RNA isolation / purification Cells - immortalized HeLa

Products Exiqon miRCURY RNA Isolation Kit

DNA isolation / purification Cells - Immortalized cell lines H1 hESc Experiment

DNA DNA isolation / purification Cells Immortalized cell lines H1 hESc

siRNA / RNAi /miRNA transfection Human Cells - Primary splenocytes Polymer / lipid Experiment

The RNA interference (RNAi) is used to inhibit gene expression or translation, by neutralizing targeted mRNA molecules. Two types of RNA molecules such as microRNA (miRNA) and small interfering RNA (siRNA) play a central role in RNAi. Few points have to considered to increase the transfection efficiency of siRNA. Always use healthy, actively dividing cells to maximize transfection efficiency. The confluency of cells should be between 50-70%. Always use the most appropriate siRNA concentration to avoid off-target effects and unwanted toxic side effects. Positive and negative controls should be used for each and every experiment to determine transfection efficiency.

RNA siRNA / RNAi /miRNA transfection Human Cells Primary splenocytes Polymer / lipid

DNA isolation / purification Cells - Immortalized cell lines Lymphoblastoid cell lines Experiment

Isolating DNA from tissues and paraffin-embedded tissue samples can be challenging as double-stranded DNA is physically fragile and highly susceptible to exo- and endonucleases. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in the presence of DNAse inhibitors. Further, extracting DNA from the nucleus need specific methods by combining physical, mechanical and chemical lysis approaches,

DNA DNA isolation / purification Cells Immortalized cell lines Lymphoblastoid cell lines

miRCURY RNA Isolation Kit Product

Get tips on using miRCURY RNA Isolation Kit to perform RNA isolation / purification Cells - immortalized MCF-7

Products Exiqon miRCURY RNA Isolation Kit

miRCURY RNA Isolation Kit Product

Get tips on using miRCURY RNA Isolation Kit to perform RNA isolation / purification Cells - immortalized MC-9

Products Exiqon miRCURY RNA Isolation Kit

Absolutely RNA FFPE Kit Product

Get tips on using Absolutely RNA FFPE Kit to perform RNA isolation / purification Tissue - rat kidney tissue

Products Agilent Technologies Absolutely RNA FFPE Kit

Cytoplasmic and Nuclear RNA Purification Kit Product

Get tips on using Cytoplasmic and Nuclear RNA Purification Kit to perform RNA isolation / purification Cells - immortalized Akata

Products Norgen Biotek Cytoplasmic and Nuclear RNA Purification Kit

Cytoplasmic and Nuclear RNA Purification Kit Product

Get tips on using Cytoplasmic and Nuclear RNA Purification Kit to perform RNA isolation / purification Cells - immortalized JY

Products Norgen Biotek Cytoplasmic and Nuclear RNA Purification Kit

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