rna-isolation-purification-tissue-mouse-cornea

Get tips on using NucleoSpin® RNA to perform RNA isolation / purification Tissue - Human Spinal cord

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

Get tips on using Absolutely RNA FFPE Kit to perform RNA isolation / purification Tissue - Human Kidney

Get tips on using Total RNA Kit, peqGOLD to perform RNA isolation / purification Tissue - Human Stomach

Get tips on using NucleoSpin® RNA XS to perform RNA isolation / purification Tissue - Human Skin

Get tips on using Ambion™ RecoverAll™ Total Nucleic Acid Isolation Kit for FFPE to perform RNA isolation / purification Tissue - rat kidney tissue

Get tips on using Gentra Puregene Mouse Tail Kit (4 g) to perform DNA isolation / purification Tissue - murine tail biopsies

Get tips on using Isolate II RNA Mini Kit to perform RNA isolation / purification Tissue - Rat Gut

Get tips on using Ambion™ RecoverAll™ Total Nucleic Acid Isolation Kit for FFPE to perform RNA isolation / purification Tissue - human kidney tissue