rna-isolation-purification-tissue-mouse-cornea

Get tips on using TRIzol™ LS Reagent to perform RNA isolation / purification Tissue - human brain tissue

Get tips on using AllPrep DNA/RNA Micro Kit to perform RNA isolation / purification Tissue - Human Blood / Serum / Plasma / Buffy coat

Get tips on using NucleoSpin® RNA to perform RNA isolation / purification Cells - primary mouse cortical neurons

Get tips on using DNeasy Blood & Tissue Kit to perform DNA isolation / purification Tissue - adipose

Get tips on using DNeasy Blood & Tissue Kit to perform DNA isolation / purification Tissue - placenta

Get tips on using DNeasy Blood & Tissue Kit to perform DNA isolation / purification Tissue - eye

Get tips on using DNeasy Blood & Tissue Kit to perform DNA isolation / purification Tissue - lung

Get tips on using DNeasy Blood & Tissue Kit to perform DNA isolation / purification Tissue - brain

Get tips on using miRNeasy FFPE Kit to perform RNA isolation / purification Tissue - rat heart muscle tissue

RNA-Seq is a method to sequence RNA by applying Next Generation Sequencing (NGS). The quality of RNA is critical for the success of RNA-Seq. The integrity of RNA is measured by the RNA integrity number (RIN). RIN is computed from RNA electrophoresis and electropherogram profiles (the peak area of the 28S rRNA should be approximately twice the peak area of the 18S rRNA). If you get the RIN value lower than 7, the possibility of getting the low quality of RNA-seq data is high. To get a high quality RNA, it is better to work with fresh samples or snap-freeze the tissues in liquid nitrogen as quickly as possible and store them at -80°C until further use. Make sure designated areas and all your filter tips, microfuge tubes, plastic, and glassware are RNase-free.