n-a

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Get tips on using N-cadherin Polyclonal Antibody to perform Western blotting N‐cadherin

Products Thermo Fisher Scientific N-cadherin Polyclonal Antibody
pFastBac-N Product

Get tips on using pFastBac-N to perform Protein Expression Eukaryotic cells - S. frugiperda AvCoV-IBV

Products Juergen A. Richt, Department of Diagnostic Medicine and Pathobio pFastBac-N

Get tips on using N-WASP siRNA (h) to perform siRNA / miRNA gene silencing Human - T47-D N-WASP

Products Santa Cruz Biotechnology N-WASP siRNA (h)

Western blotting is a widely used technique to size separate proteins from a pool of cell or tissue lysates. The technique has 4 major steps: a) gel electrophoresis, b) blocking and treatment with antigen specific antibody, c) treatment with secondary antibody and finally d) detection and visualization. Though western blotting is a widely used technique, detection of specific proteins depends on several factors, the major ones are antibody concentration, incubation time and washing steps. Key points for obtaining clean blots are: always prepare fresh buffer solutions and optimize antibody concentration. Given the advent of high-throughput protein analysis and a push to limit the use of lab consumables, onestep antibodies are developed which recognise protein of interest and also contain a detection label.

Proteins Western blotting N‐cadherin

Get tips on using Anti-N-Cadherin Antibody, clone 13A9 to perform Western blotting N‐cadherin

Products Merck Millipore Anti-N-Cadherin Antibody, clone 13A9

Get tips on using N-cadherin Antibody (8C11): sc-53488 to perform Western blotting N‐cadherin

Products Santa Cruz Biotechnology N-cadherin Antibody (8C11): sc-53488

Get tips on using ATG5 Antibody (N-term) to perform Autophagy assay cell type - RPE-J cells

Products Abgent ATG5 Antibody (N-term)

Get tips on using ATG5 Antibody (N-term) to perform Autophagy assay cell type - MEFs (mouse embryonic fibroblasts)

Products Abgent ATG5 Antibody (N-term)

Get tips on using SnoA/N siRNA (h) to perform siRNA / miRNA gene silencing Human - SW1990 SnoN

Products Santa Cruz Biotechnology SnoA/N siRNA (h)

Get tips on using Bax antibody [N1N2], N-term to perform Western blotting Bax

Products GeneTex Bax antibody [N1N2], N-term

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