3D Cell Culture Media Human breast cancer BT-549 cells-Mammospheres

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Found 3 matching solutions for this experiment

Upstream tips
You can develop chemoresistant cell lines
Protocol tips
Seed 1.0 ×104 cells/well
Use of 24-ultralow attachment plates
Analysis of spheres with a diameter >40 μm
Downstream tips
Collect spheres for other procedures, such as protein analysis
DMEM/F-12, no phenol red

Thermo Fisher Scientific

Protocol tips
Seed 1x10 3 cells/well
Protocol tips
Seed BT549 cells at a density of 2500 cells/wel/
Use of 6-well ultra-low attachment plates
Count mammospheres with diameters of ≥100 μm
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