3D Cell Culture Media Human cancer esophageal organoids

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Found 3 matching solutions for this experiment

Upstream tips
Defrost the growth factor reduced Matrigel in advance, on ice or in fridge
Protocol tips
Seed esophageal organoid units in ∼2 mL per well of growth factor reduced Matrigel, spreading over the bottom of a six-well plate
Culture for 10 days with medium changed every other day
Downstream tips
Collect organoids for microscopic analysis
Gibco™Advanced DMEM/F-12

Thermo Fisher Scientific

Upstream tips
Defrost the Matrigel in advance on ice or in fridge
Protocol tips
Seed 2000 cells per well, in 24-well plates.
Grow organoids for 11 days
Downstream tips
Fix recovered organoids (overnight in 4.0% paraformaldehyde), embedd them (2.0% Bacto-Agar: 2.5% gelatin for paraffin embedding) and subjected to histologic analyses.
Advanced DMEM

Thermo Fisher Scientific

Upstream tips
Defrost the Matrigel in advance on ice or in fridge
Protocol tips
Seed 2 × 104 cells/well of the flat bottom 24-well plates
Grow 3D organoids for 10–14 days
Downstream tips
Collect organoids for histological analysis
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