3D Cell Culture Media Human endometrium organoids

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Found 4 matching solutions for this experiment

Gibco™Advanced DMEM/F-12

Thermo Fisher Scientific

Upstream tips
Defrost the Matrigel in advance on ice or in fridge
Protocol tips
Change medium every 2-3 d
Passage of cultures every 7-10 d
Downstream tips
Organoids can be frozen for further derivation of cultures
DMEM/F-12

Thermo Fisher Scientific

Upstream tips
Defrost the growth factor reduced Matrigel in advance, on ice or in fridge
Protocol tips
Refresh medium every 2 days
Passage organoids every 14 days
DMEM/F-12, no phenol red

Thermo Fisher Scientific

Upstream tips
Defrost the Matrigel in advance on ice or in fridge
Protocol tips
You can proceed with co-cultures of organoids and stromal cells from the same individual
To prevent organoids from sedimenting on the insert filter, use Matrigel with sufficiently high protein content (>10 mg/ml)
DMEM/F-12, no phenol red

Thermo Fisher Scientific

Upstream tips
Defrost the Matrigel in advance on ice or in fridge
Protocol tips
You can proceed with co-cultures of organoids and stromal cells from the same individual
To prevent organoids from sedimenting on the insert filter, use Matrigel with sufficiently high protein content (>10 mg/ml)
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