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Found 4 matching solutions for this experiment
| Upstream tips |
Protocol tips |
Downstream tips |
| Defrost the Matrigel in advance on ice or in fridge |
Change medium every 2-3 d
Passage of cultures every 7-10 d
|
Organoids can be frozen for further derivation of cultures |
| Upstream tips |
| Defrost the Matrigel in advance on ice or in fridge |
| Protocol tips |
Change medium every 2-3 d
Passage of cultures every 7-10 d
|
| Downstream tips |
| Organoids can be frozen for further derivation of cultures |
| Upstream tips |
Protocol tips |
Downstream tips |
| Defrost the growth factor reduced Matrigel in advance, on ice or in fridge |
Refresh medium every 2 days
Passage organoids every 14 days |
|
| Upstream tips |
| Defrost the growth factor reduced Matrigel in advance, on ice or in fridge |
| Protocol tips |
Refresh medium every 2 days
Passage organoids every 14 days |
| Upstream tips |
Protocol tips |
Downstream tips |
| Defrost the Matrigel in advance on ice or in fridge |
You can proceed with co-cultures of organoids and stromal cells from the same individual
To prevent organoids from sedimenting on the insert filter, use Matrigel with sufficiently high protein content (>10 mg/ml)
|
|
| Upstream tips |
| Defrost the Matrigel in advance on ice or in fridge |
| Protocol tips |
You can proceed with co-cultures of organoids and stromal cells from the same individual
To prevent organoids from sedimenting on the insert filter, use Matrigel with sufficiently high protein content (>10 mg/ml)
|
| Upstream tips |
Protocol tips |
Downstream tips |
| Defrost the Matrigel in advance on ice or in fridge |
You can proceed with co-cultures of organoids and stromal cells from the same individual
To prevent organoids from sedimenting on the insert filter, use Matrigel with sufficiently high protein content (>10 mg/ml)
|
|
| Upstream tips |
| Defrost the Matrigel in advance on ice or in fridge |
| Protocol tips |
You can proceed with co-cultures of organoids and stromal cells from the same individual
To prevent organoids from sedimenting on the insert filter, use Matrigel with sufficiently high protein content (>10 mg/ml)
|
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