3D Cell Culture Media Human gastric organoids

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Found 3 matching solutions for this experiment

Gibco™Advanced DMEM/F-12

Thermo Fisher Scientific

Upstream tips
Defrost the Matrigel in advance on ice or in fridge
Protocol tips
Use of pre-warmed 24-well plate
Add fresh medium was every 2-3 days.
Downstream tips
Collect organoids to fix them in 4% paraformaldehyde for 1hr, embed in 2% agarose gel, or directly fix in the matrigel in formalin for generation of paraffin blocks, sectioning and staining
DMEM/F-12

Thermo Fisher Scientific

Upstream tips
Defrost the Matrigel in advance on ice or in fridge
Protocol tips
Harvest organoids after 4–7 days of growth
Upstream tips
Defrost the Matrigel in advance on ice or in fridge
Protocol tips
Change medium every 4 days
Organoid passage every 2–3 days
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