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Found 3 matching solutions for this experiment
|Defrost the Matrigel in advance on ice or in fridge|
|Use of pre-warmed 24-well plate
Add fresh medium was every 2-3 days.
|Collect organoids to fix them in 4% paraformaldehyde for 1hr, embed in 2% agarose gel, or directly fix in the matrigel in formalin for generation of paraffin blocks, sectioning and staining|