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Found 3 matching solutions for this experiment
| Upstream tips |
Protocol tips |
Downstream tips |
| Defrost the Matrigel in advance on ice or in fridge |
Replace culture medium every 2 days |
After removing the culture medium, RNA can be extracted with Trizol. For immunofluorescence, organoids can be fixed with 4% PFA for 30 min, permeabilized, and blocked for non-specific binding with 3% BSA prior to incubation with primary antibodies. |
| Upstream tips |
| Defrost the Matrigel in advance on ice or in fridge |
| Protocol tips |
| Replace culture medium every 2 days |
| Downstream tips |
| After removing the culture medium, RNA can be extracted with Trizol. For immunofluorescence, organoids can be fixed with 4% PFA for 30 min, permeabilized, and blocked for non-specific binding with 3% BSA prior to incubation with primary antibodies. |
| Upstream tips |
Protocol tips |
Downstream tips |
| Defrost the Matrigel in advance on ice or in fridge |
Change medium every second or third day
Culture organoids for five to seven days |
Organoids can be cryo conserved at −150 °C |
| Upstream tips |
| Defrost the Matrigel in advance on ice or in fridge |
| Protocol tips |
Change medium every second or third day
Culture organoids for five to seven days |
| Downstream tips |
| Organoids can be cryo conserved at −150 °C |
| Upstream tips |
Protocol tips |
Downstream tips |
| Defrost the Matrigel in advance on ice or in fridge |
Use of 48-well plates |
|
| Upstream tips |
| Defrost the Matrigel in advance on ice or in fridge |
| Protocol tips |
| Use of 48-well plates |
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