3D Cell Culture Media Human small intestinal organoids

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Found 3 matching solutions for this experiment

Upstream tips
Defrost the Matrigel in advance on ice or in fridge
Protocol tips
Replace culture medium every 2 days
Downstream tips
After removing the culture medium, RNA can be extracted with Trizol. For immunofluorescence, organoids can be fixed with 4% PFA for 30 min, permeabilized, and blocked for non-specific binding with 3% BSA prior to incubation with primary antibodies.
DMEM/F-12

Thermo Fisher Scientific

Upstream tips
Defrost the Matrigel in advance on ice or in fridge
Protocol tips
Change medium every second or third day
Culture organoids for five to seven days
Downstream tips
Organoids can be cryo conserved at −150 °C
Gibco™Advanced DMEM/F-12

Thermo Fisher Scientific

Upstream tips
Defrost the Matrigel in advance on ice or in fridge
Protocol tips
Use of 48-well plates
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