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Found 2 matching solutions for this experiment
| Upstream tips |
Protocol tips |
Downstream tips |
| Defrost the Matrigel in advance on ice or in fridge |
Change media every 3-4 days and after 12 days organoids harvest organoids for further processing |
|
| Upstream tips |
| Defrost the Matrigel in advance on ice or in fridge |
| Protocol tips |
| Change media every 3-4 days and after 12 days organoids harvest organoids for further processing |
| Upstream tips |
Protocol tips |
Downstream tips |
| Defrost the Matrigel in advance on ice or in fridge |
Refresh medium was refreshed every 2 days
Passage of cultures every 7-10 days at a ratio of 1:4 to 1:8 (depending on the medium used, determining the growth efficiency)
|
|
| Upstream tips |
| Defrost the Matrigel in advance on ice or in fridge |
| Protocol tips |
Refresh medium was refreshed every 2 days
Passage of cultures every 7-10 days at a ratio of 1:4 to 1:8 (depending on the medium used, determining the growth efficiency)
|
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