3D Cell Culture Media Mouse endometrium organoids

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Found 2 matching solutions for this experiment

Upstream tips
Defrost the Matrigel in advance on ice or in fridge
Protocol tips
Change media every 3-4 days and after 12 days organoids harvest organoids for further processing
DMEM/F-12

Thermo Fisher Scientific

Upstream tips
Defrost the Matrigel in advance on ice or in fridge
Protocol tips
Refresh medium was refreshed every 2 days
Passage of cultures every 7-10 days at a ratio of 1:4 to 1:8 (depending on the medium used, determining the growth efficiency)
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