3D Cell Culture Media Mouse liver organoids

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Found 3 matching solutions for this experiment

Gibco™Advanced DMEM/F-12

Thermo Fisher Scientific

Upstream tips
Defrost the Matrigel in advance on ice or in fridge
Protocol tips
Seeding of ciliary ducts or sorted cells
William's E Medium, no phenol red

Thermo Fisher Scientific

Upstream tips
Defrost the growth factor reduced Matrigel in advance, on ice or in fridge
Protocol tips
Seed 1000 cells per 20μL of matrigel droplets for primary cultures and 500 – 2000 cells per 20 μL drops for passage
DMEM/F-12

Thermo Fisher Scientific

Upstream tips
Defrost the growth factor reduced Matrigel in advance, on ice or in fridge
Protocol tips
Replace growth factors every other day and Add fresh media every four days.
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