3D Cell Culture Media Mouse primary breast ephitelial cells-Mammospheres

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Found 3 matching solutions for this experiment

EpiCult™-B Mouse Medium Kit

STEMCELL technologies

Upstream tips
Use freshly sorted cells
Protocol tips
Seed 4,000 cells per well at a density of 8,000 viable cells/mL
Use low attachment plates
DMEM/F-12

Thermo Fisher Scientific

Protocol tips
Seed 4 × 104 cells/ml to an ultra-low attachment tray
Protocol tips
Culture isolated MECs in MEC culture medium for 1 day, before mammosphere culture
Seed single MECs at a density of 25 000 viable cells/ml
Use of ultralow attachment plates
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