3D Cell Culture Media Mouse small intestinal organoids

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Found 3 matching solutions for this experiment

Upstream tips
Defrost the Matrigel in advance on ice or in fridge
Protocol tips
Optional: co-culture of mouse intestinal epithelial cells and isolated fibroblasts
Gibco™Advanced DMEM/F-12

Thermo Fisher Scientific

Upstream tips
Defrost the Matrigel in advance on ice or in fridge
Protocol tips
After 2 days in culture remove CHIR and Rock inhibitor
Upstream tips
Defrost the growth factor reduced Matrigel in advance, on ice or in fridge
Protocol tips
Seed different volumes of organoid units to assess survival rate and growth
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