3D Cell Culture Media Murina esophageal organoids

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Found 2 matching solutions for this experiment

Upstream tips
Defrost the growth factor reduced Matrigel in advance, on ice or in fridge
Protocol tips
Seed esophageal organoid units in ∼2 mL per well of growth factor reduced Matrigel, spreading over the bottom of a six-well plate
Culture for 10 days with medium changed every other day
Downstream tips
Collect organoids for microscopic analysis
Advanced DMEM

Thermo Fisher Scientific

Upstream tips
Defrost the Matrigel in advance on ice or in fridge
Protocol tips
Seed 2 × 104 cells/well of the flat bottom 24-well plates
Grow 3D organoids for 10–14 days
Downstream tips
Collect organoids for histological analysis
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