3D Cell Culture Media Murina esophageal organoids

Start discussion

No discussions found

Start your discussion

Share your thoughts or question with experts in your field

Start a discussion

Found 2 matching solutions for this experiment

Upstream tips
Defrost the growth factor reduced Matrigel in advance, on ice or in fridge
Protocol tips
Seed esophageal organoid units in ∼2 mL per well of growth factor reduced Matrigel, spreading over the bottom of a six-well plate
Culture for 10 days with medium changed every other day
Downstream tips
Collect organoids for microscopic analysis
Advanced DMEM

Thermo Fisher Scientific

Upstream tips
Defrost the Matrigel in advance on ice or in fridge
Protocol tips
Seed 2 × 104 cells/well of the flat bottom 24-well plates
Grow 3D organoids for 10–14 days
Downstream tips
Collect organoids for histological analysis
Can't find the product you've used to perform this experiment? It would be great if you can help us by Adding a product!
Become shareholder Discussions About us Contact Privacy Terms