Acid phosphatase assay cell type - murine macrophage cells

Acid phosphatase detection heavily relies on determining the concentration of tartrate-resistant acid phosphatase (TRAP) in the sample. Hence, sample preparation is very crucial and it should be done strictly as per kit manufacturer instructions to avoid any inconsistency and poor sensitivity.

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Immunodiagnosticsystems (ids)

Upstream tips
- Cell were grown with α-MEM containing RANKL (40 ng/ml) and the medium is changed and the supernatant is collected every 2 days (3, 5 and 7 days) and stored at -80C
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