Angiogenesis assay human - HMVEC (human microvascular lung endothelial cells)

A standard angiogenic assay involves the autonomous endothelial cell response of self-organization into microvessels, also known as tubes when seeded on a basement membrane matrix in the presence of the appropriate growth factors. However, the component of basement membrane matrix may also affect the tube formation by endothelial cells. Hence it is important to use a standard angiogenesis assay kit or use the same membrane matrix with known composition to standardize the assay conditions.

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2 years ago

2 years ago by Sander J. Mikkelsen Denmark

endothelial cells vs HUVEC cells?

What is the difference between endothelial cells and HUVEC cells for angiogenesis assay?

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Protocol tips
- Cells were seeded in ECG medium with or without 100 ng/mL human rIL-9.

- After seeding cells on ECMatrix it is incubated for 48h and assayed after 24 and 48h.
Protocol tips
- The pretreated cells plus RA SF (1:20) or flagellin (10 ng/ml) were added to the polymerized matrigel and the plate was incubated for 16h at 37°C
Protocol tips
- After seeding for 24h cells are treated with Galectin-3 for another 24h.
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