Apoptosis assay cell type - BxPC-3 human pancreatic cancer cell line

A gross majority of classical apoptotic attributes can be quantitatively examined by flow cytometry, the preferred platform for rapid assessment of multiple cellular attributes at a single-cell level. However, sample preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

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Found 3 matching solutions for this experiment

Protocol tips
Add 4 μl Annexin-V FITC and 4 μl propidium iodide was and incubate for 20 minutes in dark
Protocol tips
Incubate the cells in the DNA-labeling solution for 60 minutes at 36°C in a temperature
controlled bath
Protocol tips
Add 5 µl of PE Annexin V and 5 µl 7-AAD and incubate for 15 minutes in dark
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