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|- 5 × 10^4 cells/well were seeded.
- 24h post seeding, cells were treated with MINS for 24 h, 36 h or 48 h.
|- First cells were stained with propidium iodide (50 μg/mL) in the dark for 60 min at 4˚C.
- Next stained with Annexin V-FITC in the dark for 15 min at room temperature.
- Lastly, cells were stained with Hoechst 33342 (10 µg/mL)
in the dark for 15 min at room temperature