Apoptosis assay cell type - HUVEC

A gross majority of classical apoptotic attributes can be quantitatively examined by flow cytometry, the preferred platform for rapid assessment of multiple cellular attributes at a single-cell level. However, sample preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

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Found 3 matching solutions for this experiment

Upstream tips
- Cells were treated with or without 5, 10 and 20 μM of HMJ-30 for 24 h
Downstream tips
- Non specific labelling can be corrected by fixing cells by treating with 4% buffer PFA or Formalin.

- When Nonspecific labelling is present, reduce concentration of TdT
- When high background is present reduce concentration of labelling mix by 50%
Upstream tips
- Cells were cultured in 35 mm2 disks
Protocol tips
- After treating cells with Annexin V and PI working solution they are incubated with Annexin-binding buffer on ice
Protocol tips
- Treated with FITC Annexin V and PI and incubated for 15 min at RT (25°C) in the dark
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