Apoptosis assay cell type - L02 human hepatocyte cell line

A gross majority of classical apoptotic attributes can be quantitatively examined by flow cytometry, the preferred platform for rapid assessment of multiple cellular attributes at a single-cell level. However, sample preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

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Found 2 matching solutions for this experiment

Upstream tips
- Store all components at 4-8℃ and protect from light.

- The Annexin V-FITC can be stored at -20℃ for longer use.
Protocol tips
- After adding Annexin V-FITC and PI Staining Solution cells were Incubated at room temperature for 10 min and protect from light.
Protocol tips
- Treated with FITC Annexin V and PI and incubated for 15 min at RT (25°C) in the dark
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