Apoptosis assay cell type - OECM-1 human oral squamous cell carcinoma

A gross majority of classical apoptotic attributes can be quantitatively examined by flow cytometry, the preferred platform for rapid assessment of multiple cellular attributes at a single-cell level. However, sample preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

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Found 2 matching solutions for this experiment

Upstream tips
- 2 × 10^4 cells/well were seededfor assay.

- cells were treated with LPLI
Protocol tips
- Cells are stained with PI and annexin V-FITC and incubated on ice for 10–15 min
Upstream tips
- 1 × 10^6 cells/mL were used for the assay.
Protocol tips
- Treated with FITC Annexin V and PI and incubated for 15 min at RT (25°C) in the dark
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