No discussions found
Start your discussion
Share your thoughts or question with experts in your field
Start a discussion
Found 2 matching solutions for this experiment
Upstream tips |
Protocol tips |
Downstream tips |
|
OVCAR-3, A2780/CP70 and IOSE-364 cells were seeded in 24-well plates at 1 × 105 cells/well and incubated overnight. Cells were treated with various concentrations (0–4 μM) of ChK for 24 h. After treatment, cells were stained with 10 μg/mL Hoechst 33342 (Sigma, St. Louis, MO) in PBS for 10 min in the dark at 37 °C. Cell apoptosis was examined under a fluorescence microscope (ZEISS), and data were collected from three independent experiments. |
|
Protocol tips |
OVCAR-3, A2780/CP70 and IOSE-364 cells were seeded in 24-well plates at 1 × 105 cells/well and incubated overnight. Cells were treated with various concentrations (0–4 μM) of ChK for 24 h. After treatment, cells were stained with 10 μg/mL Hoechst 33342 (Sigma, St. Louis, MO) in PBS for 10 min in the dark at 37 °C. Cell apoptosis was examined under a fluorescence microscope (ZEISS), and data were collected from three independent experiments. |
Upstream tips |
Protocol tips |
Downstream tips |
|
Add 5 µl of FITC Annexin V and 5 µl PI and incubate for 15min at RT. |
|
Protocol tips |
Add 5 µl of FITC Annexin V and 5 µl PI and incubate for 15min at RT. |
Can't find the product you've used to perform this experiment? It would be great if you can help us by
Adding a product!