Apoptosis assay cell type - OVCAR-3 human ovarian cancer

A gross majority of classical apoptotic attributes can be quantitatively examined by flow cytometry, the preferred platform for rapid assessment of multiple cellular attributes at a single-cell level. However, sample preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Start discussion

No discussions found

Start your discussion

Share your thoughts or question with experts in your field

Start a discussion

Found 3 matching solutions for this experiment

Annexin V-FITC Kit

Miltenyibiotec

Protocol tips
After
incubation for 48 h, the cells were washed with PBS,
trypinsized, and stained with fluorescein isothycanateconjugated annexin V using Annexin V-FITC kit
(Miltenyi Biotec, Germany) and propidium iodide
(PI), following the manufacturer’s protocol.
Upstream tips
- 1 × 10^5 cells/well were seeded.
Protocol tips
- Cells were treated with various concentrations (0–4 μM) of ChK for 24 h.

- Hoechst 33342 in PBS for 10 min in the dark at 37 °C.
Upstream tips
- 1×10^4 cells/ml were used for the assay.

- Treated with 25 µM myricetin for 48 h at 37°C
Can't find the product you've used to perform this experiment? It would be great if you can help us by Adding a product!
Become shareholder Discussions About us Contact Privacy Terms