Apoptosis assay cell type - PC-3 human prostate cancer

A gross majority of classical apoptotic attributes can be quantitatively examined by flow cytometry, the preferred platform for rapid assessment of multiple cellular attributes at a single-cell level. However, sample preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

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Found 3 matching solutions for this experiment

Upstream tips
- 1.0 x 106 cells were seeded.
Protocol tips
- Incubate arrays with sample at 40C overnight.

- Incubation may be done for 2 hours at room temperature.
Downstream tips
- avoid solution flowing into neighboring wells for better reading
Upstream tips
- Cells treated with sorafenib (0–4 μM) alone or in combination with other agent.
Protocol tips
- Treated with FITC Annexin V and PI and incubated for 15 min at RT (25°C) in the dark
Upstream tips
- Cells were treated with different concentrations
of vitexicarpin for 24h after overnight culture.
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