Apoptosis assay cell type - RPMI-8226 human myeloma

A gross majority of classical apoptotic attributes can be quantitatively examined by flow cytometry, the preferred platform for rapid assessment of multiple cellular attributes at a single-cell level. However, sample preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

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Found 2 matching solutions for this experiment

Upstream tips
- Cells were pretreated and incubated with SC-560, NS-398, U46619, and SQ29548 or transfected with TP small interfering RNA (siRNA) for 24 h
Downstream tips
- Both FITC-labeled Annexin V and PI are light sensitive. All staining procedures must be performed without direct exposure to intense light.
Upstream tips
- 1×10^6 cells/ml cells were seeded
Protocol tips
- Cells were treated with annexin V–Alexa Fluor® 488 and incubated in the dark for 15 min at 37 ◦C.
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