Apoptosis assay cell type - Sao2-2, MG-62 human osteosarcoma

A gross majority of classical apoptotic attributes can be quantitatively examined by flow cytometry, the preferred platform for rapid assessment of multiple cellular attributes at a single-cell level. However, sample preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

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Found 4 matching solutions for this experiment

Upstream tips
- 1 × 10^6 cells per well were seeded onto the 100 mm culture dishes.

- Treated with various concentrations of bryostatin for 12 h.

- It was followed by H2O2 (100 µM) for 48h.
Protocol tips
- Treated with FITC Annexin V and PI and incubated for 20 min at RT (25°C) in the dark
Upstream tips
- Cells were pretreated with NC or miR-133a mimics respectively.
Protocol tips
- After 48h , cells wereassayed for apoptosis.

- Cells were treated with YO-PRO®-1 stock solution and PI stock solution and incubated for for 20–30 minutes on ice.
Upstream tips
- Prepare 1· Binding Buffer by diluting 1 ml of the 10· Binding Buffer with 9 ml of deionized water.

- Dissolve the staurosporine in DMSO to a concentration
of 100 mg/ml
Protocol tips
- cells were collected after 48 h of culture.
Upstream tips
- Cells are treated with 50 nM of oleandrin for 0, 24 and 48 h.
Protocol tips
- Always stain cells with Annexin before fixation (makes cell membrane leaky)
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