Apoptosis assay cell type - SH-SY5Y human derived cells bone marrow

A gross majority of classical apoptotic attributes can be quantitatively examined by flow cytometry, the preferred platform for rapid assessment of multiple cellular attributes at a single-cell level. However, sample preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

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Found 3 matching solutions for this experiment

Upstream tips
- 12 × 104 cells/well were platted in 6 well plate.

- Cells were exposed to to MPP+ (1000 μM), α-mangostin (10 μM), or the combination of MPP+ (1000 μM) and α-mangostin (10 μM) for 24 hours.
Protocol tips
- Cells were incubated withFITC annexin-V and PI at room temperature for 15 minutes
Upstream tips
- Use identical cell numbers and volumes for the assay and the negative control samples.

- Cells were cultured in normoxic and hypoxic conditions.
Downstream tips
- The plates were incubated at room temperature and luminescence was measured at time 0, 30 and 60 min after the start of the assay,
Upstream tips
- 1 × 10^6 cells/ml. were used for assay.
Protocol tips
- Annexin V and propidium iodide (PI) were added and incubated in dark for 15 minutes

- Cells were fixed, washed and treated with RNase and incubated for 15 minutes at 37°C.
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