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Thermo Fisher Scientific
|- 12 × 104 cells/well were platted in 6 well plate.
- Cells were exposed to to MPP+ (1000 μM), α-mangostin (10 μM), or the combination of MPP+ (1000 μM) and α-mangostin (10 μM) for 24 hours.
|- Cells were incubated withFITC annexin-V and PI at room temperature for 15 minutes|
|- Use identical cell numbers and volumes for the assay and the negative control samples.
- Cells were cultured in normoxic and hypoxic conditions.
|- The plates were incubated at room temperature and luminescence was measured at time 0, 30 and 60 min after the start of the assay,|