Apoptosis assay cell type - SKOV3

A gross majority of classical apoptotic attributes can be quantitatively examined by flow cytometry, the preferred platform for rapid assessment of multiple cellular attributes at a single-cell level. However, sample preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

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Found 3 matching solutions for this experiment

Cell-APOPercentage™ Apoptosis Assay

Biocolor

Upstream tips	Protocol tips	Downstream tips
- 3 × 10^4 were seeded in 96 well plate.	- Cells were cultured in culture medium with and without fresh medium containing 5% FBS (as control) or 5% ascite in presence or not of H2O2 for 6 hours before adding APOPercentage Dye.	- Wells were shaken for 20 minutes before being read at 540 nm

Upstream tips
- 3 × 10^4 were seeded in 96 well plate.
Protocol tips
- Cells were cultured in culture medium with and without fresh medium containing 5% FBS (as control) or 5% ascite in presence or not of H2O2 for 6 hours before adding APOPercentage Dye.
Downstream tips
- Wells were shaken for 20 minutes before being read at 540 nm

Manufacturer protocol Publication protocol 1 Relevant paper

Cytochrome c Releasing Apoptosis Assay Kit

Abcam

Upstream tips	Protocol tips	Downstream tips
- Cells were pre treated with artonin E at different time points	- Cells were Dounce-homogenized before centrifuging at 7,000×g for 10 min at 4°C.

Upstream tips
- Cells were pre treated with artonin E at different time points
Protocol tips
- Cells were Dounce-homogenized before centrifuging at 7,000×g for 10 min at 4°C.

Manufacturer protocol Publication protocol 1 Relevant paper

FITC Annexin V Apoptosis Detection Kit with 7-AAD

BioLegend

Upstream tips	Protocol tips	Downstream tips
- Do not freeze components	- Add FITC Annexin V and 7-AAD Viability Staining Solution and incubate for 15 min at RT.

Upstream tips
- Do not freeze components
Protocol tips
- Add FITC Annexin V and 7-AAD Viability Staining Solution and incubate for 15 min at RT.

Manufacturer protocol Publication protocol 1 Relevant paper

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