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|- Cells were treated with LfcinB-P13 (range, 0–60 µg/ml) for 24 h.|
|- Treated with AV, PI and incubate 10-15 minutes at room temperature.|
|- Due to the calcium dependence of the Annexin V:PS interaction, it is critical to avoid buffers containing EDTA or other calcium chelators during
Annexin V experiments.
- Annexin V can only be used as a marker of apoptosis in cells where the plasma membrane is intact because destroying the
integrity of the plasma membrane will allow non-specific binding of Annexin V to PS inside the cell.
|The cell apoptosis was measured by flow cytometry
analysis with an Annexin-V/FITC and propidium iodide
(PI) apoptosis detection kit (BioVision, USA), according to the manufacturer’s standards. Briefly, after 48 h
of transfection, the cells were collected and suspended
in annexin-binding buffer and exposed to Annexin-VFITC and PI for 15 min in the dark at room temperature. The apoptotic percentage of cells was quantified by