Cell Culture Contamination Detection Kit Mycoplasma

Contamination can affect cell characteristics, i.e., growth, metabolism, and morphology leading to unreliable and erroneous experimental data. Depending on the source of contaminants, one can detect contamination by using a light microscope, gram stain, isothermal amplification, or PCR. Bacteria and fungi can usually be identified by optical microscopy. Mycoplasma in cell cultures cannot be detected visually. Hence, these microbes can go unnoticed for long periods and are determined using dedicated assays. Early and rapid identification of contaminants is vital to detect, handle and prevent contamination for good cell-culture practices. However, detection and identification can be challenging and tricky based on usual visual identifications. Hence it is essential to use a standard contamination detection kit to detect and maintain best practices.

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Found 19 matching solutions for this experiment

Upstream tips
• Lonza’s MycoAlert® Mycoplasma Detection Assays are selective biochemical tests to detect Mycoplasma contamination in cell cultures.
Protocol tips
Detection of Mycoplasma in cell cultures is performed as follows: • Viable mycoplasma in a test sample (cell culture supernatant) are lysed
• The released mycoplasmal enzymes react with the MycoAlert® Substrate
• The ATP is then transferred into a light signal via the luciferase enzyme in the MycoAlert® Reagent.
• Measuring the level of ATP in a sample both before (read A) and after the addition of the MycoAlert® Substrate (read B), a ratio can be determined that indicates the presence or absence of mycoplasma.
Upstream tips
• Detection of cell culture contaminating mycoplasma by MycoStrip™ is based on isothermal PCR.
•MycoStrip™ has been specifically designed to detect the Mycoplasma and Acholeplasma species that most commonly contaminate cell culture. These include the six species that account for 95% of all contaminations: M. orale, M. hyorhinis, M. arginini, M. fermentans, M. hominis, and A. laidlawii.
Protocol tips
• MycoStrip™ is able to detect as low as 10-102 CFU/ml and thus, can be used to detect mycoplasma contamination before it significantly affects experimental results, which typically occurs at ~107 CFU/ml.
Upstream tips
• The Resistance Plus MG FleXible assay is a CE-IVD multiplex PCR assay that targets the MgPa adhesin gene for the detection of M. genitalium and four 23S rRNA mutations associated with macrolide resistance.
Protocol tips
• Prior to loading onto the GeneXpert system, the reagent chamber of the FleXible cartridge was filled with 44ml of MG FleXible reaction mix, and 1 ml of the specimen plus 10ml of internal control cells were added to the sample chamber, according to the manufacturer’s instructions
Downstream tips
• Analyze data using GeneXpert Dx system version 5.1 software
Upstream tips
• The Universal Mycoplasma Detection Kit offers a quick and sensitive PCR-based test to detect mycoplasma contaminants in cell culture.
• The kit detects over 60 species of Mycoplasma, Acholeplasma, Spiroplasma and Ureaplasma including the eight species most likely to afflict cell cultures: M. arginini, M. fermentans, M. hominis, M.hyorhinis, M. orale, M. pirum, M. salivarium, and A. laidlawii.
Protocol tips
• Prepare a 3% agarose gel.
• Prepare samples: Add 10 µL of the PCR product to 1.5 µL loading buffer
MycoSEQ™ Mycoplasma Detection Kit

Thermo Fisher Scientific

Upstream tips
• The MycoSEQ Mycoplasma Detection Kit is a real-time PCR assay designed and validated to meet the requirements of European Pharmacopeia Chapter 2.6.7.
• The assay's sensitivity has been demonstrated by internal and external validations to detect 10 cfu/mL or the genomic equivalent of 10 GC/mL as recommended by regulatory guidance.
Protocol tips
• PCR Method: qPCR
• Detection Method: SYBR
Downstream tips
• Anlayze samples with AccuSEQ™ Software v3.1 on the QuantStudio™ 5 Instrument.
Upstream tips
• In the PrepSEQ 1-2-3 kit protocol, a rapid procedure recovers DNA from total lysate of the test sample using magnetic-bead based technology.
Protocol tips
• The PrepSEQ 1-2-3 kit extracts Mycoplasma genomic DNA from research cell lines, stem cell lines, and cellular and tissue therapy samples with high efficiency.
• The protocol is recommended for use with a starting sample volume of 100 µL with a maximum cell density of 106 cells.
Upstream tips
• The MycoFluor™ Mycoplasma Detection Kit provides an ultrasensitive, rapid, and simple fluorescence microscopic assay for the visual identification of mycoplasma infection in laboratory cell cultures.
Protocol tips
• In order to detect mycoplasma, the fluorescent MycoFluor™ reagent is added directly to the culture medium, with or without cells, and the stained sample is then examined under a fluorescence microscope.
Upstream tips
• The LookOut Mycoplasma PCR Detection Kit is highly sensitive, with a detection limit of only 2 genomes per μl.
•Taking advantage of the highly specific JumpStart Taq DNA Polymerase and a proprietary primer design, false positives are greatly reduced.
Protocol tips
• Technique: PCR
• The reaction tubes included with the kit are pre-coated with appropriate dNTPs, primers, and loading dye.
Upstream tips
• Highly-sensitive and -specific PCR assay designed to test for the presence of over 90 species of Mycoplasma, Acholeplasma, and Spiroplasma in cell cultures with a detection limit of 10 CFU/mL.
Protocol tips
• The EZ-PCR™ Mycoplasma Detection Kit includes an internal amplification control, a plasmid containing a non-mycoplasma-specific DNA sequence to test for false negatives.
Upstream tips
• PlasmoTest™ features two major components:
• The mycoplasma sensor cells, HEK-Blue™-2 cells
- the HEK-Blue™ Detection medium
Protocol tips
• Sensitivity: detects 5.102-5.105 cfu/ml depending on the species of mycoplasma
• No false positive: a positive result indicates the presence of a cell culture contaminant
Upstream tips
• They exploit the activity of mycoplasmal enzymes which are found in the vast majority of about 200 mycoplasma species, but are not present in eukaryotic cells.
Protocol tips
• The positive control for the MycoAlert® Mycoplasma Detection Kit is available separately in the MycoAlert® Assay Control Set.
Upstream tips
• abm's Mycoplasma PCR Detection Kit (Cat. No. G238) offers highly specific and sensitive detection of 200+ strains of Mycoplasmas in less than 2 hours.
• Kit is able to detect as low as 10 copies of Mycoplasma /sample
Protocol tips
• Positive control is included in the kit
Upstream tips
• The MycoTOOL Mycoplasma Real-Time PCR Kit is designed to test at least 10 cell culture samples for the absence of Mycoplasma.
Protocol tips
• Automated workflow using automated sample preparation and Real-Time PCR
• <5 hours, including sample preparation (data on file)
cobas® ️TV/MG

Roche Lifesciences

Upstream tips
• cobas® TV/MG for use on the cobas® 6800/8800 Systems is an automated, qualitative in vitro diagnostic test, that utilizes real-time polymerase chain reaction (PCR), for the direct detection of Trichomonas vaginalis (TV) and/or Mycoplasma genitalium (MG) DNA
Upstream tips
• MYCOPLASMA IST 3 combines enumeration, identification and antibiotic susceptibility testing for detection of Mycoplasma hominis and Ureaplasma spp.

• Complete diagnosis in one step
• Simple, secure and convenient
Upstream tips
• CE-IVD-marked multiplex real-time PCR assay that detects M.genitalium (undisclosed target) and six mutations in the 23S rRNA gene associated with macrolide resistance
Downstream tips
• Analyze data using Seegene viewer software version 1.6
Upstream tips
• Detect mycoplasma contamination in cell culture supernatants, media, and supplements with the next generation MycoAlert™ Kit allowing the use of less sensitive plate luminometers or multifunctional readers.
Upstream tips
• Detection requires as little as 1–5fg of mycoplasma DNA corresponding to 2–5 mycoplasma per sample volume.
Protocol tips
• Does not include Taq Polymerase. Optimized for use with D9307, Taq DNA Polymerase
Upstream tips
• M. genitalium - 23S rRNA gene; 5 mutations: A2058G, A2059G, A2058T, A2058C, A2059C, associated with macrolide-resistance by melt-curve analysis
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