Cell Culture Contamination Detection Kit Virus

Contamination can affect cell characteristics, i.e., growth, metabolism, and morphology leading to unreliable and erroneous experimental data. Depending on the source of contaminants, one can detect contamination by using a light microscope, gram stain, isothermal amplification, or PCR. Bacteria and fungi can usually be identified by optical microscopy. Mycoplasma in cell cultures cannot be detected visually. Hence, these microbes can go unnoticed for long periods and are determined using dedicated assays. Early and rapid identification of contaminants is vital to detect, handle and prevent contamination for good cell-culture practices. However, detection and identification can be challenging and tricky based on usual visual identifications. Hence it is essential to use a standard contamination detection kit to detect and maintain best practices.

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Found 17 matching solutions for this experiment

Upstream tips
• Kit detects and differentiates Parainfluenza virus types 1, 2 and 3 antigens directly in nasopharyngeal aspirates or in cell culture preparations.
• Use IMAGEN Parainfluenza Kit as a rapid, sensitive, and specific method for detection and differentiation of Parainfluenza virus types 1, 2, and 3 antigens
Protocol tips
• Specimens are incubated with the FITC conjugated reagents for 15 minutes.
• Excess reagent is then removed by washing with phosphate-buffered saline (PBS).
• The stained areas are mounted and viewed using epifluorescence illumination.
Downstream tips
• Parainfluenza virus types 1, 2, or 3 are present then characteristic apple-green intracellular fluorescence is seen within infected cells, which contrasts with the red background staining of uninfected cells.
Upstream tips
• Kit is designed for the rapid and sensitive quantitation of influenza neuraminidase inhibitor resistance in dilutions of virus culture medium in a 96-well microplate format.
Protocol tips
• Detection Method: Enhanced Chemiluminescence
• Simply incubate your virus samples with dilutions of neuraminidase inhibitor, add NA-Star chemiluminescent substrate, incubate again, and then add the accelerator solution, which triggers light emission from the reaction product.
Upstream tips
• Assay is part of an integrated DNA purification and real-time PCR-based system for rapid, sensitive detection of MMV from a wide range of sample types.
• The ViralSEQ MMV assay detects the four known strains of MMV: i, p, m, and c, including seven genomic sequences reported in the NCBI database.
Protocol tips
• Sensitivity: ≥ 10 copies MMV DNA
• Detection Method: Primer-Probe Detection
• GC-Rich PCR Performance: High
• PCR Method: qPCR
Upstream tips
• Detect and differentiate Influenza A and B virus in clinical samples with Thermo Scientific™ IMAGEN™ Influenza Virus A and B Kit.
• Includes: 2 x positive control slide, mounting fluid, Influenza A virus test reagent, Influenza B virus test reagent, instruction for use.
Protocol tips
• Detectable Analytes: Influenza - Epitopes of Influenza virus glycoproteins and fusion proteins specific to either Influenza virus type A or Influenza virus type B.
Upstream tips
• Kit detect respiratory syncytical virus (RSV), influenza A and B virus, parainfluenza virus 1,2 & 3 and adenovirus in respiratory specimens.
Protocol tips
• The assay cannot differentiate between the viruses. Individual viruses should be further identified and confirmed using monospecific FITC labeled monoclonal antibody reagents.
• Use IMAGEN Respiratory Virus Screen Kit as a rapid, sensitive, and specific method for the presumptive detection
Upstream tips
• A direct immunofluorescence test for the detection and typing of HSV 1 and HSV 2 in cell cultures.
• A rapid, sensitive and specific method for the detection and differentiation of HSV1 and HSV2 isolates in cell culture monolayers.
Protocol tips
• Detectable Analytes: HSV glycoproteins specific to either HSV1 or HSV 2.
• Includes: 2 x 2 positive control slide, mounting fluid, HSV type 1 reagent, HSV type 2 reagent, instruction for use.
Upstream tips
• Kit enables the simultaneous detection and differentiation of RNA from dengue virus (DENV), chikungunya virus (CHIKV), and Zika virus (ZIKV) in urine and serum.
• It is a lyophilized, multiplex, one-step real-time PCR.
Protocol tips
• GC-Rich PCR Performance: Low
• No. of Reactions: 96 Reactions
• Detection Method: Primer-Probe Detection
• Detection Method: Primer-Probe Detection
Upstream tips
• 1 ready-to-use mix that includes a set of oligonucleotides for detection of the target gene.
• 1 external positive control of the same nature as the target & 1 internal positive control
Protocol tips
• Detection Method: Primer-Probe Detection
• Sample Type: Abortive Animal: Whole Blood (EDTA tube), spleen, Spleen
• Kit Type: Bluetongue Virus Real Time PCR Kit
Upstream tips
• Kit is a single-well duplex real-time PCR for the detection of African Swine Fever Virus (ASFV) DNA in serum, whole blood, and tissue samples of pigs using TaqMan primer and probes.
Protocol tips
• The kit is sensitive and specific for the detection of ASFV DNA, allowing for reliable and rapid screening during outbreaks and monitoring situations.
Upstream tips
• Designed for the rapid and sensitive quantitation of influenza neuraminidase inhibitor resistance in dilutions of virus culture medium in a 96-well microplate format.
Protocol tips
• Detection Method: Enhanced Chemiluminescence
• For Use With: Luminometer
• Product Type: Neuraminidase Assay
Upstream tips
• detection of sf-rhabdovirus from the Sf9/Sf21 baculovirus platform used in the development of gene therapies, cell-based vaccines, and similar biotherapeutics.
Protocol tips
• This kit is bundled together with sample preparation reagents in the form of a PrepSEQ Nucleic Acid Sample Preparation Kit
ViralSEQ™ Vesivirus Detection Kit

Thermo Fisher Scientific

Upstream tips
• The ViralSEQ™ Vesivirus Detection Assay is a PCR-based test that enables users to quickly isolate and detect Vesivirus 2117 and all other known strains with high sensitivity and specificity.
Protocol tips
• Detection Method: Primer-Probe Detection
• PCR Method: 1-Step qRT-PCR
Upstream tips
• Kit is intended for use in the rapid, in vitro detection of bovine virus diarrhea virus (BVDV RNA) extracted from ear notches in acute and persistently infected (PI) cattle.
Protocol tips
• The kit is not intended for discriminating viral subtypes
• GC-Rich PCR Performance
High
VetMAX™ West Nile Virus Kit

Thermo Fisher Scientific

Upstream tips
• Real-time PCR enables sensitive and specific detection of pathogen nucleic acid in animal samples, allowing for reliable and rapid screening and detection of infected animals.
Protocol tips
• Detection Method: Primer-Probe Detection
• GC-Rich PCR Performance: Low
Upstream tips
• A qualitative immunofluorescence test for the detection and identification of Herpes simplex virus types I and II (HSV-I and HSV-II) directly from clinical samples or after growth in tissue culture.
Upstream tips
• This direct immunofluorescence assay utilizes three monoclonal antibodies in order to detect specific structural proteins expressed in all strains of human Respiratory Syncytial Virus.
VetMAX™ Schmallenberg Virus Kit

Thermo Fisher Scientific

Upstream tips
• TaqMan™ probes - RNA - duplex - One well reaction
Protocol tips
• 1 ready-to-use mix that includes a set of oligonucleotides for detection of the target gene
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