Cell cycle assay human - A2780

Cell cycle can be challenging due to difference introduced by sample handling, timing, and difference within the sample. Downstream instriuments to analyse cell cycle (Multicolor flow cytometry and multicolor imaging) can answer these challenges. Relevant markers can be combined with cell phenotyping markers to look at events within subpopulations of cells.

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Found 3 matching solutions for this experiment

Upstream tips
- After cells were seeded, they were treated with control or SP-6-27 treated (0.5 uM, 24h)
Protocol tips
- PI staining was performed for 10 min at 4°C
FxCycle™ Far Red Stain

Thermo Fisher Scientific

Protocol tips
- Cells were incubated for 60 min in the presence of 10 μM 5-ethynyl-2’-deoxyuridine (EdU) at 37°C.
Upstream tips
- Cells were analysed for cell cycle after 72h cell culture
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