Cell cycle assay human - BxPC-3

Cell cycle can be challenging due to difference introduced by sample handling, timing, and difference within the sample. Downstream instriuments to analyse cell cycle (Multicolor flow cytometry and multicolor imaging) can answer these challenges. Relevant markers can be combined with cell phenotyping markers to look at events within subpopulations of cells.

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Found 3 matching solutions for this experiment

Protocol tips
- Gambogic acid is added to cells at varying concentrations (0.0, 0.5, 1.0, and 2.0 μM) for 24 h.

- Cells were fixed by 75% ethanol. Incubated with RNaseA at 37 °C in a water bath for 40 min and PI solution for 20 min in dark
Upstream tips
- Cells were seeded and treated with vehicle control, 100nM free paclitaxel, 15ug/mL α-CEA-PTX, or 15ug/mL α-CEA-680-PTX (both at 100nM effective paclitaxel) for 24 hours.
Protocol tips

Downstream tips
- If low level of staining is noticed, increase volume of stain used.
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