Cell cycle assay human - MCF-7

Cell cycle can be challenging due to difference introduced by sample handling, timing, and difference within the sample. Downstream instriuments to analyse cell cycle (Multicolor flow cytometry and multicolor imaging) can answer these challenges. Relevant markers can be combined with cell phenotyping markers to look at events within subpopulations of cells.

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Found 3 matching solutions for this experiment

Upstream tips
It is important to have a single cell suspension prior to ethanol fixation
Protocol tips
Resuspend the cell pellet in 200 μL of Muse™ Cell Cycle Reagent.
Upstream tips
- After cells are seeded and treated with vernodalin for 24 hours, cells were fixed with 70% ethanol overnight.
Upstream tips
- Cels were seeded for 24 hours, and incubated in the absence or presence of erucin for an another 24 hours,
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