Cell cycle assay human - SKOV3

Cell cycle can be challenging due to difference introduced by sample handling, timing, and difference within the sample. Downstream instriuments to analyse cell cycle (Multicolor flow cytometry and multicolor imaging) can answer these challenges. Relevant markers can be combined with cell phenotyping markers to look at events within subpopulations of cells.

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Found 3 matching solutions for this experiment

Upstream tips
- Cells were cultured, seeded and later centrifuged at 450 × g for 5 minutes, and fixed with ethanol.
Downstream tips
- The red fluorescence was detected by a flow cytometer at an excitation wavelength of 488 nm.
Upstream tips
- This assay was used for determining the percentage of S-phase cells
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