Cell cycle assay human - SW480

Cell cycle can be challenging due to difference introduced by sample handling, timing, and difference within the sample. Downstream instriuments to analyse cell cycle (Multicolor flow cytometry and multicolor imaging) can answer these challenges. Relevant markers can be combined with cell phenotyping markers to look at events within subpopulations of cells.

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Found 3 matching solutions for this experiment

Protocol tips
- Cells were pretreated with incubated with dimethyl sulfoxide, sulindac (30, 60 and 120 µM), atorvastatin (0.1, 0.5, 1 µM) or Sul/Atorva.

- Cells were fixed with 70% ethanol overnight.
Upstream tips
- Cells are seeded and treated with UV-C and incubated with/without DMEM 10 μM of cisplatin for 96 h.
Protocol tips
- Cell fixation, washing, and cell cycle staining should be done in 12 x 75-mm polystyrene tubes
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