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Found 3 matching solutions for this experiment
| Upstream tips |
Protocol tips |
Downstream tips |
| Donor lymphocytes isolated as T and B cells (EasySep HLA T Cell Enrichment Kit and EasySep HLA B Cell Enrichment Kit, StemCell Technologies, Vancouver, Canada) were added separately to the wells of a Terasaki plate (1 × 105 1 μL buffer) together with 1 μL recipient sera. |
The plate was incubated for 40 minutes at 20°C to 25°C followed by a further 70 minutes after adding 5 μL rabbit complement (Bio-Rad). |
Then the samples were visualized with 3 μL of eosin and fixed with 5 μL of formalin. The result was acquired by visual inspection under a phase-contrast microscope. |
| Upstream tips |
| Donor lymphocytes isolated as T and B cells (EasySep HLA T Cell Enrichment Kit and EasySep HLA B Cell Enrichment Kit, StemCell Technologies, Vancouver, Canada) were added separately to the wells of a Terasaki plate (1 × 105 1 μL buffer) together with 1 μL recipient sera. |
| Protocol tips |
| The plate was incubated for 40 minutes at 20°C to 25°C followed by a further 70 minutes after adding 5 μL rabbit complement (Bio-Rad). |
| Downstream tips |
| Then the samples were visualized with 3 μL of eosin and fixed with 5 μL of formalin. The result was acquired by visual inspection under a phase-contrast microscope. |
| Upstream tips |
Protocol tips |
Downstream tips |
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Following manufacturer's instructions |
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| Protocol tips |
| Following manufacturer's instructions |
| Upstream tips |
Protocol tips |
Downstream tips |
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Human B cells were purified first with a RosetteSep HLA B cell Enrichment Cocktail (StemCell Technologies) through negative selection followed by incubation with anti-CD27–coupled magnetic beads (Miltenyi Biotec) to isolate memory B (CD27+) cells. |
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| Protocol tips |
| Human B cells were purified first with a RosetteSep HLA B cell Enrichment Cocktail (StemCell Technologies) through negative selection followed by incubation with anti-CD27–coupled magnetic beads (Miltenyi Biotec) to isolate memory B (CD27+) cells. |
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