Cell Isolation HLA B Cell

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Found 3 matching solutions for this experiment

Upstream tips
Donor lymphocytes isolated as T and B cells (EasySep HLA T Cell Enrichment Kit and EasySep HLA B Cell Enrichment Kit, StemCell Technologies, Vancouver, Canada) were added separately to the wells of a Terasaki plate (1 × 105 1 μL buffer) together with 1 μL recipient sera.
Protocol tips
The plate was incubated for 40 minutes at 20°C to 25°C followed by a further 70 minutes after adding 5 μL rabbit complement (Bio-Rad).
Downstream tips
Then the samples were visualized with 3 μL of eosin and fixed with 5 μL of formalin. The result was acquired by visual inspection under a phase-contrast microscope.
Protocol tips
Following manufacturer's instructions
Protocol tips
Human B cells were purified first with a RosetteSep HLA B cell Enrichment Cocktail (StemCell Technologies) through negative selection followed by incubation with anti-CD27–coupled magnetic beads (Miltenyi Biotec) to isolate memory B (CD27+) cells.
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