Cell migration / Invasion cell type - MCF7

Cell Invasion or Cell Migration assays are technically challenging to set up as the gradient between the two compartments equilibrates in time during the assay. It is also problematic to view cells and for cells to migrate through a non-physiologic polycarbonate or polypropylene filter. Care must be taken while loading the well with cells to form a single cell suspension. Precaution must be taken while trypsinization (under-trypsinization can lead to cell clumping while over-trypsinization could strip off adhesion molecules necessary for migration). This leads to difficulty in getting significant results, when only small numbers of cells cross the filter or when the distribution and/or staining of the cells is uneven.

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Found 3 matching solutions for this experiment

Upstream tips
Seed 1000 cells in 24-well filter-inserts,
Protocol tips
Cover plate and incubate for 8 hours at 37°C in a CO2 incubator (4-6% CO2).
Protocol tips
For migration assay incubate cells 2–3 h at 5% CO2 and 37 °C
Upstream tips
Seed 70 μl of 5 × 105 cells/ml
Protocol tips
Incubate at 37°C and 5 % CO2 for 24 h
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