Cell migration / Invasion cell type - MG-63

Cell Invasion or Cell Migration assays are technically challenging to set up as the gradient between the two compartments equilibrates in time during the assay. It is also problematic to view cells and for cells to migrate through a non-physiologic polycarbonate or polypropylene filter. Care must be taken while loading the well with cells to form a single cell suspension. Precaution must be taken while trypsinization (under-trypsinization can lead to cell clumping while over-trypsinization could strip off adhesion molecules necessary for migration). This leads to difficulty in getting significant results, when only small numbers of cells cross the filter or when the distribution and/or staining of the cells is uneven.

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Found 3 matching solutions for this experiment

Protocol tips
Starve cells by incubating 18-24 hours prior to assay in appropriate serumfree medium (DMEM, EMEM, or equivalent).

Cover plate and incubate for 24 - 72 hours at 37°C in a CO2 incubator (4-6% CO2).
Protocol tips
Incubate the Chambers for 24 hours in a humidified tissue culture incubator, at 37C, 5% CO2 atmosphere.
Upstream tips
Seed 1x10^6 cells
Protocol tips
Incubate cells for 48 hours at 37ºC in 5% CO2 atmosphere.
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