Cell migration / Invasion cell type - Skov3

Cell Invasion or Cell Migration assays are technically challenging to set up as the gradient between the two compartments equilibrates in time during the assay. It is also problematic to view cells and for cells to migrate through a non-physiologic polycarbonate or polypropylene filter. Care must be taken while loading the well with cells to form a single cell suspension. Precaution must be taken while trypsinization (under-trypsinization can lead to cell clumping while over-trypsinization could strip off adhesion molecules necessary for migration). This leads to difficulty in getting significant results, when only small numbers of cells cross the filter or when the distribution and/or staining of the cells is uneven.

Start discussion

No discussions found

Start your discussion

Share your thoughts or question with experts in your field

Start a discussion

Found 3 matching solutions for this experiment

Protocol tips
Add 10% fetal bovine serum) to the lower chamber.

Cover plate and incubate for 24 - 72 hours at 37°C in a CO2 incubator (4-6% CO2).
Protocol tips
Incubate for 24-48 hours at 37ºC in 5% CO2 atmosphere.
Downstream tips
Measure the OD 560nm in a
plate reader
Upstream tips
Seed 2.5×10^4 cells
Protocol tips
Incubate chamber at 37 oC in CO2 incubator for 24 hours
Can't find the product you've used to perform this experiment? It would be great if you can help us by Adding a product!
Become shareholder Discussions About us Contact Privacy Terms