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Found 2 matching solutions for this experiment
|-Add protease inhibitors to all lysis solutions before use.
|-Keep cells on ice between the rounds of homogenisations.
- Increase or decrease the homogenization step to maximize the yield of nuclei depending on cell line.
|-Use 10 µl per ChIP
-Avoid over fixation.
-purchase expensive, "hing end" sonicator to get reproducible shearing.
-Avoid repeated freeze/thaw cycles.
-Keep all reagents on ice when not in storage.
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