DNA Damage Assay A549

DNA damage assay is a standard method for determining in-vivo/in-vitro genotoxicity by measuring the breaks in the DNA chain of animal and plant cells. Initial DNA damage leads to cell cycle arrest and, at the final stages, leads to induction of senescence or cell death (apoptosis, necrosis, autophagy, or mitotic catastrophe). Detection of DNA damage from mild to moderate to severe is challenging when studying genotoxicity in the pool of cells. It is favorable to use DNA damage assay kits available for prominent identification of the extent of damage in the analysis.

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Found 3 matching solutions for this experiment

Upstream tips
Seed 1–2 × 10^4 cells/ml mixed in 1% low fusion point agarose and then place on a slide
Downstream tips
The length of tail, the percentage of DNA in the tail of each comet can be measured using CASP software (Version 1.2.3; available at http://casp.sourceforge.net)
Upstream tips
Seed 2×10^5 cells/ml
Downstream tips
DNA damage can be analyzed using the IMSTAR Pathfinder system
Upstream tips
Seed 2x10^5 cells/mL
Downstream tips
Use NIH ImageJ software for quantification of comet tail
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