DNA Damage Assay HEK 293T

DNA damage assay is a standard method for determining in-vivo/in-vitro genotoxicity by measuring the breaks in the DNA chain of animal and plant cells. Initial DNA damage leads to cell cycle arrest and, at the final stages, leads to induction of senescence or cell death (apoptosis, necrosis, autophagy, or mitotic catastrophe). Detection of DNA damage from mild to moderate to severe is challenging when studying genotoxicity in the pool of cells. It is favorable to use DNA damage assay kits available for prominent identification of the extent of damage in the analysis.

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Found 4 matching solutions for this experiment

OxiSelect™ Comet Assay Kit (3-Well Slides)

Cell Biolabs

Upstream tips	Protocol tips	Downstream tips
Seed 1x10^5 cells/ml		The DNA damage is quantified by measuring the displacement between the genetic material of the nucleus ('comet head') and the resulting 'tail'. Tail Moment and Tail DNA% are the two most common parameters to analyze Comet assay results. At least 50 -100 cells should be analyzed per sample. The Tail Moment has been suggested to be an appropriate index of induced DNA damage in considering both the migration of the genetic material as well as the relative amount of DNA in the tail.

Upstream tips
Seed 1x10^5 cells/ml
Downstream tips
The DNA damage is quantified by measuring the displacement between the genetic material of the nucleus ('comet head') and the resulting 'tail'. Tail Moment and Tail DNA% are the two most common parameters to analyze Comet assay results. At least 50 -100 cells should be analyzed per sample. The Tail Moment has been suggested to be an appropriate index of induced DNA damage in considering both the migration of the genetic material as well as the relative amount of DNA in the tail.

Manufacturer protocol Publication protocol 1 Relevant paper

One-step TUNEL Assay Kit

Elabscience

Upstream tips	Protocol tips	Downstream tips
Seed 2X10^5 cells/ml/well

Upstream tips
Seed 2X10^5 cells/ml/well

Publication protocol 1 Relevant paper

CometAssay Electrophoresis System II

Bio-Techne

Upstream tips	Protocol tips	Downstream tips
Cell suspension at a concentration of 10^5cells/ml was mixed in a 1:1 ratio withTrevigen LMAgarose (#4250-050-02) at 37◦C.		The images of the comets were analyzed using CellProfiler software (version 2.1.1 rev 6c2d896).

Upstream tips
Cell suspension at a concentration of 10^5cells/ml was mixed in a 1:1 ratio withTrevigen LMAgarose (#4250-050-02) at 37◦C.
Downstream tips
The images of the comets were analyzed using CellProfiler software (version 2.1.1 rev 6c2d896).

Manufacturer protocol Publication protocol 1 Relevant paper

CometAssay Single Cell Gel Electrophoresis Assay

Bio-Techne

Upstream tips	Protocol tips	Downstream tips
1 × 10^5 cells/ml were embedded in LM Agarose (1:10)	The slide was treated with lysis solution, alkali, and subjected to alkaline electrophoresis.

Upstream tips
1 × 10^5 cells/ml were embedded in LM Agarose (1:10)
Protocol tips
The slide was treated with lysis solution, alkali, and subjected to alkaline electrophoresis.

Manufacturer protocol Publication protocol 1 Relevant paper

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