DNA Damage Assay Saos-2

DNA damage assay is a standard method for determining in-vivo/in-vitro genotoxicity by measuring the breaks in the DNA chain of animal and plant cells. Initial DNA damage leads to cell cycle arrest and, at the final stages, leads to induction of senescence or cell death (apoptosis, necrosis, autophagy, or mitotic catastrophe). Detection of DNA damage from mild to moderate to severe is challenging when studying genotoxicity in the pool of cells. It is favorable to use DNA damage assay kits available for prominent identification of the extent of damage in the analysis.

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Found 3 matching solutions for this experiment

Upstream tips
Seed 2X10^4 cells in 100ml cell culture medium for 96-well microplates and allowed to adhere for 24h.
Downstream tips
Samples can be measured within 5 min after adding the stop solution at a wavelength of 450 nm on a Bio-Rad microplate reader with 690 nm as reference.
Upstream tips
Seed 50,000 cells per well in a 96-well plate
Downstream tips
Comets can be imaged by using the LionHeart FX automated microscope (Biotek) and the Gen5 Image Prime software to construct image montages that were analyzed using TriTek CometScore 2.0.0.38.
Upstream tips
Seed 5x10^3 cells/well in 24-well plates
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