DNA Damage Assay SJSA-1

DNA damage assay is a standard method for determining in-vivo/in-vitro genotoxicity by measuring the breaks in the DNA chain of animal and plant cells. Initial DNA damage leads to cell cycle arrest and, at the final stages, leads to induction of senescence or cell death (apoptosis, necrosis, autophagy, or mitotic catastrophe). Detection of DNA damage from mild to moderate to severe is challenging when studying genotoxicity in the pool of cells. It is favorable to use DNA damage assay kits available for prominent identification of the extent of damage in the analysis.

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Found 3 matching solutions for this experiment

Upstream tips
Seed 2x10^5 cells/ml
Downstream tips
Quantization of the DNA in the tails of the comets can be performed with Quantity One software (Bio-Rad Laboratories) and ImageJ software version 1.49 (http://rsbweb.nih.gov/ij/index.html).
Upstream tips
Seed 1.5 X 10^5 cells/ml
Upstream tips
Seed 50,000 cells per well in a 96-well plate
Downstream tips
Comets can be imaged by using the LionHeart FX automated microscope (Biotek) and the Gen5 Image Prime software to construct image montages that were analyzed using TriTek CometScore 2.0.0.38.
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