DNA quantification Human - HeLa

Though DNA quantification is but one small step in the multifaceted DNA sample preparation workflow, it can have large implications on the performance and validity of conclusions drawn from downstream assays. Major challenges include accuracy, precision, reproducibility, and detection of present contamination. Among UV spectrophotometry, fluorescence and real-time PCR based methods, the quantification method should be chosen based on the requirement of the downstream assay.

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4 years ago

4 years ago by Dorota Ostrowska Poland

About nuclease-free water in DNA quantification

Hi everyone! I have a question about DNA quantification. Do I need to use nuclease-free water for DNA quantification and are there any repercussions if I don’t use any?

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Found 3 matching solutions for this experiment

Hoechst 33342

Thermo Fisher Scientific

Protocol tips
Place aluminum foil over
the sample to protect it from light and incubate at room temperature for 5-10 minutes.

Optimize incubation time to
minimize signal bleeding through to other channels.
Qubit dsDNA HS Assay Kit

Thermo Fisher Scientific

Upstream tips
Do not label the side of the tube as this could interfere with the sample read.
Protocol tips
Do not vortex while adding and mixing of solutions A, B and C
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