DNA quantification Mouse - NIH3T3

Though DNA quantification is but one small step in the multifaceted DNA sample preparation workflow, it can have large implications on the performance and validity of conclusions drawn from downstream assays. Major challenges include accuracy, precision, reproducibility, and detection of present contamination. Among UV spectrophotometry, fluorescence and real-time PCR based methods, the quantification method should be chosen based on the requirement of the downstream assay .

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Found 3 matching solutions for this experiment

Protocol tips
To minimize photo bleaching
effects, keep the time for fluorescence measurement constant for all samples.

During incubation at room temperature, protected from light.
Upstream tips
Exposure to
light will reduce the sensitivity of the assay. Store QuantiFluor®
dsDNA Dye and working solution protected from light
Qubit dsDNA HS Assay Kit

Thermo Fisher Scientific

Upstream tips
Do not label the side of the tube as this could interfere with the sample read.
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